Laser-assisted hatching of cleavage-stage embryos impairs developmental potential and increases DNA damage in blastocysts

Honguntikar, Sachin D and Uppangala, Shubhashree and Salian, Sujith R and Kalthur, Guruprasad and Kumar, Pratap and Adiga, Satish Kumar (2014) Laser-assisted hatching of cleavage-stage embryos impairs developmental potential and increases DNA damage in blastocysts. Lasers in Medical Science, 29 (4). pp. 1-9. ISSN 0268-8921

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Abstract

This study aims to investigate the influence of two- (day 2) and six-to-eight-cell-stage (day 3) laserassisted hatchings on the developmental potential and genetic integrity of the embryos. In this prospective experimental study, two- and six-to-eight-cell-stage mouse embryos were subjected to laser hatching using 1,480 nm diode laser, and then assessed for the developmental potential and DNA integrity in blastocysts. Similarly, fourcell- stage human embryos from 20 patients were also subjected to laser hatching, and then assessed for the developmental competence. Laser-assisted hatching in mouse embryos significantly enhanced the blastocyst hatching potential on day 4.5 (P<0.0001). However, a significant decline in blastocyst total cell number (TCN) was observed in six-to-eight-cell-stage laser-hatched embryos (P<0.001). Conversely, no significant difference in TCN was observed between laser-hatched and unhatched human four-cell-stage embryos after 24 h. Attempt to understand the genetic integrity in laser-hatched mouse blastocysts revealed significantly higher labeling index when hatching was done at two- (P<0.01) and six-toeight- cell stage (P<0.05). DNA damage induced by the laser manipulation may affect implantation and postimplantation developmental potential of the embryos. However, further studies are required to elucidate the impact of laser-induced DNA damage on the reproductive outcome.

Item Type: Article
Uncontrolled Keywords: Laser-assisted hatching; DNA damage; preimplantation; blastocyst.
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 10 Jul 2014 04:43
Last Modified: 10 Jul 2014 04:43
URI: http://eprints.manipal.edu/id/eprint/140061

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