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Total phenolic content and free radical scavenging activity of Pterocarpus marsupium heartwood & Tribulus terrestris dry fruits: An in vitro comparative study

*, Bheemesh Vangalapati and Manjrekar, Poornima and Hegde, Anupama (2014) Total phenolic content and free radical scavenging activity of Pterocarpus marsupium heartwood & Tribulus terrestris dry fruits: An in vitro comparative study. Journal of Pharmacy Research, 8 (5). pp. 610-613. ISSN 0974-6943

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Abstract

Background: Majority of the disorders like diabetes, atherosclerosis, cancer and neurodegenerative diseases are linked to oxidative stress. Antioxidants derived from plant sources offer resistance against the oxidative stress by way of their free radicals scavenging & lipid peroxidation inhibitory actions. Present study was designed to compare the free radical scavenging activity in relation to the total phenolic content of P. marsupium heartwood & T. terrestris dry fruits. Methods: Total phenolic content of the extracts was determined by using Folin-Ciocalteu method following a slightly modified method of Singleton et al by comparing with standard Gallic acid. The ability to scavenge 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) radical is measured by a decrease in the absorbance at 517 nm as described by Hou et al comparing with standard Ascorbic acid. Results: Phenolic contents ranging from 45 to 160 and 5 to 25mg of Gallic acid equivalents (GAE) per gram of extract were present in P. marsupium& T. terrestris respectively. At 100μg concentration P. marsupium& T. terrestris produced 85.9 & 67.5% of DPPH scavenging activity respectively and the standard ascorbic acid(10μg) produced 90% scavenging activity. Conclusion: The present study concludes that both the plant extracts have shown significant in vitro free radical scavenging activity. Among them, aqueous extract of P. marsupium heartwood exhibited better activity which can be attributed to the presence of high phenolic content. Hence these herbal constituents are of adjuvant importance in the management of various free-radical mediated pathological conditions.

Item Type: Article
Uncontrolled Keywords: Oxidative stress, free radical scavenging, DPPH, P. marsupium, T. terrestris
Subjects: Medicine > KMC Mangalore > Biochemistry
Depositing User: KMCMLR User
Date Deposited: 01 Aug 2014 10:30
Last Modified: 01 Aug 2014 10:30
URI: http://eprints.manipal.edu/id/eprint/140300

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