Modulatory effect of spondias pinnata bark extract on intestinal MPO & sucrase activities in etoposide induced rat model for mucositis

Reddy , Sudarshan C and Shetty, Beena V and Rao, Gayathri M (2014) Modulatory effect of spondias pinnata bark extract on intestinal MPO & sucrase activities in etoposide induced rat model for mucositis. International Journal of Biological & Pharmaceutical Research, 5 (5). pp. 396-402. ISSN 0976 - 3651

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Abstract

Chemotherapeutic agents kill not only cancer cells, but also the rapidly replicating intestinal epithelial cells, causing intestinal mucositis. This study was under taken with a view to elucidate the possible protective potentials of S.pinnata bark extract on mucositis prompted by etoposide. Rat mucositis model was developed by injecting single dose of etoposide (i.p.) and treated with S.pinnata bark extract (100 & 200 mg/ kg body wt.) for next 72 hrs. Treatment efficacy was determined by changes in the intestinal morphology and biochemical parameters after 72 hr., with and without intervention. Histology of small intestine (H&E stain) and parameters incorporating intestinal GSH, Sucrase, NO and MPO on top of progressions in sustenance and water admission and body weight were contemplated. There was a significant decrease in reduced glutathione and sucrase levels and a significant increase in NO and MPO activities in intestinal tissue after etoposide injection. However, in the post treatment groups (in both 100 & 200 mg), reduced glutathione and sucrase levels reverted back to that of normal. NO levels reverted to normal levels in response to 100mg/kg body wt. of S.pinnata extract; however it remained high in the group treated with 200mg/kg body wt. MPO levels showed least response to the intervention during the study. Histological studies showed a good response to S.pinnata bark extract intervention. The results suggest that S.pinnata bark extract has some potential to prevent the toxic effects of etoposide which expedites to mucositis.

Item Type: Article
Uncontrolled Keywords: Etoposide, Mucositis, S.pinnata, Sucrase, MPO.
Subjects: Medicine > KMC Mangalore > Biochemistry
Depositing User: KMCMLR User
Date Deposited: 14 Aug 2014 06:57
Last Modified: 14 Aug 2014 06:57
URI: http://eprints.manipal.edu/id/eprint/140374

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