A novel method for blood volume estimation using trivalent chromium in rabbit models

Baby, Prathap Moothamadathil and Kumar, Pramod and Kumar, Rajesh and Jacob, Sanu S and Binu, VS and Karun, Kalesh M (2014) A novel method for blood volume estimation using trivalent chromium in rabbit models. Indian Journal of Plastic Surgery, 47 (2). pp. 242-248. ISSN 0970-0358

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Background: Blood volume measurement though important in management of critically illpatients is not routinely estimated in clinical practice owing to labour intensive, intricate and time consuming nature of existing methods. Aims: The aim was to compare blood volume estimations using trivalent chromium [51Cr(III)] and standard Evans blue dye (EBD) method in New Zealand white rabbit models and establish correction-factor (CF). Materials and Methods: Blood volume estimation in 33 rabbits was carried out using EBD method and concentration determined using spectrophotometric assay followed by blood volume estimation using direct injection of 51Cr(III). Twenty out of 33 rabbits were used to find CF by dividing blood volume estimation using EBD with blood volume estimation using 51Cr(III). CF is validated in 13 rabbits by multiplying it with blood volume estimation values obtained using 51Cr(III). Results: The mean circulating blood volume of 33 rabbits using EBD was 142.02 ± 22.77 ml or 65.76 ± 9.31 ml/kg and using 51Cr(III) was estimated to be 195.66 ± 47.30 ml or 89.81 ± 17.88 ml/kg. The CF was found to be 0.77. The meanblood volume of 13 rabbits measured using EBD was 139.54 ± 27.19 ml or 66.33 ± 8.26 ml/kg and using 51Cr(III) with CF was 152.73 ± 46.25 ml or 71.87 ± 13.81 ml/kg (P = 0.11). Conclusions: The estimation of blood volume using 51Cr(III) was comparable to standard EBD method using CF. With further research in this direction, we envisage human blood volume estimation using 51Cr(III) to find its application in acute clinical settings.

Item Type: Article
Uncontrolled Keywords: Blood volume; Evans blue dye; measurement; trivalent chromium.
Subjects: Departments at MU > Statistics
Medicine > MMMC Manipal > Physiology
Medicine > KMC Manipal > Nuclear Medicine
Medicine > KMC Manipal > Physiology
Medicine > KMC Manipal > Plastic Surgery
Depositing User: KMC Manipal
Date Deposited: 18 Aug 2014 07:39
Last Modified: 18 Aug 2014 07:39
URI: http://eprints.manipal.edu/id/eprint/140386

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