Urea hydrolysis in saturated loam soil

Bhat, Ramananda M and Murthy, D V R and Saidutta, M B (2011) Urea hydrolysis in saturated loam soil. ARPN journal of agricultural and biological science, 6 (3). pp. 60-63. ISSN 1990-6145

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In order to evaluate the extent of loss of applied fertilizer urea due to hydrolysis catalyzed by the enzyme urease and assist in the development of mathematical model for the spread of urea from the source of application, it is necessary to conduct hydrolysis studies in wet soils. Agricultural loam soil, with 13% clay content from west coast region of India, was taken up for this purpose. Maximum particle size of the soil was restricted to 2 mm in the study. Batch trials were conducted with different urea concentrations maintained in the soil which was previously incubated at 270C for 48 hours under saturated condition. Evolved gases were allowed to escape to prevent build up of alkali and the subsequent deactivation of the enzyme at high pH. Urea estimation was carried out by colorimetric method. Results indicate that for soil solution urea concentration up to 43.6 mg/mL, the rate of hydrolysis increased with increasing initial urea concentration. For concentrations in the range of 43.6 to 243 mg/mL, rate of hydrolysis decreased with increasing initial urea concentration. The urease induced hydrolysis was completely deactivated at concentration of 305 mg/mL and beyond, due to the substrate induced inhibition. The experimental data could be fitted to a substrate inhibition model.

Item Type: Article
Uncontrolled Keywords: loam soils, enzyme, hydrolysis, urea.
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 20 Aug 2014 09:54
Last Modified: 20 Aug 2014 09:54
URI: http://eprints.manipal.edu/id/eprint/140401

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