Evaluation and optimization of food-grade tannin acyl hydrolase production by a probiotic Lactobacillus plantarum strain in submerged and solid state fermentation

Kannan, N and Rajendran, Aravindan (2012) Evaluation and optimization of food-grade tannin acyl hydrolase production by a probiotic Lactobacillus plantarum strain in submerged and solid state fermentation. Food and Bioproducts processing, 90 (4). pp. 780-792.

[img] PDF
Food.pdf - Published Version
Restricted to Registered users only

Download (1MB) | Request a copy

Abstract

Tannin acyl hydrolase (Tannase) production by Lactobacillus plantarum MTCC1407 was studied in submerged and solid-state fermentation process. Sequential optimization strategy using Plackett-Burman screening and response surface methodology was adopted to optimize the submerged fermentation process. Eight medium components were evaluated initially by Plackett-Burman two level factorial designs to identify the most significant parameters that affect the tannase production. The significant variables affecting tannase production were found to be tannic acid, glucose and MnSO4.7H2O. These factors were further optimized by response surface methodology. Maximum tannase activity of 9.13 U ml-1 was observed at 30 h using the following medium composition (g l-1): tannic acid, 13.16; glucose, 1.5; NH4Cl, 1.0; CaCl2.2H2O, 1.0; K2HPO4, 0.5; KH2PO4, 0.5; MgSO4.7H2O, 0.5 and MnSO4.7H2O, 0.03. Among the various carbon sources examined for tannase production by Lactobacillus plantarum, glucose and tannic acid combination was found to be decisive for enhancing tannase yield. Solid state fermentation was conducted using various solid substrates and agricultural residues. Maximum tannase activity of 5.319 U gds-1 was obtained using coffee husk as substrate. Unstructured kinetic models, logistic model and Luedeking-Piret model were used to describe the cell mass and tannase production respectively in submerged fermentation.

Item Type: Article
Uncontrolled Keywords: Tannase; Plackett–Burman experimental design; Response surface methodology; Solid state fermentation; Unstructured kinetic modeling
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 04 Sep 2014 08:48
Last Modified: 06 Sep 2014 06:07
URI: http://eprints.manipal.edu/id/eprint/140542

Actions (login required)

View Item View Item