Methyl Parathion Inhibits the Nuclear Maturation, Decreases the Cytoplasmic Quality in Oocytes and Alters the Developmental Potential of Embryos of Swiss Albino Mice

Nair, Ramya and Singha, Vikram Jeet and Salian, Sujith Raj and Kalthur, Sneha Guruprasad and D’Souza, Antony Sylvan and Shetty, Pallavi K and Mutalik, Srinivas and Kalthur, Guruprasad and Adiga, Satish Kumar (2014) Methyl Parathion Inhibits the Nuclear Maturation, Decreases the Cytoplasmic Quality in Oocytes and Alters the Developmental Potential of Embryos of Swiss Albino Mice. Toxicology and Applied Pharmacology, 279 (3). pp. 338-350.

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Abstract

Methyl parathion (MP) is one of themost commonly used and extremely toxic organophosphorous group of pesticide. A large number of studies in the literature suggest that it has adverse effects on the male reproductive system. However, there is limited information about its toxicity to the female reproductive system. In the present study we report the toxic effects of methyl parathion on the female reproductive system using Swiss albino mice as the experimental model. The female mice were administered orally with 5, 10 and 20 mg/kg of MP. One week later, the mice were superovulated with pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) to study the quality of the oocytes, spindle organization, developmental potential of early embryos and the DNA integrity in blastocysts. MP exposure resulted in a non-significant decrease in the number of primordial follicles and increased DNA damage in granulosa cells. ThoughMP did not have any effect on the ovulation it had a significant inhibito ry effect on the nuclear maturity of oocytes which was associated with spindle deformity. In addition, the oocytes had higher cytoplasmic abnormalities with depleted glutathione level. Even though it did not have any effect on the fertilization and blastocyst rate at lower doses, at 20mg/kgMP it resulted in a significant decrease in blastocyst hatching, decrease in cell number and high DNA damage. While low body weight gain was observed in F1 generation from 5 mg/kg group, at higher dose, the body weight in F1 generation was marginally higher than control. Post-natal death in F1 generation was observed only in mice treated with 20 mg/kg MP. In conclusion, we report that MP has adverse effects on the oocyte quality, developmental potential of the embryo and reproductive outcome.

Item Type: Article
Uncontrolled Keywords: Methyl parathion; Oocyte;Embryo; DNA damage; Spindle damage; Mitochondrial distribution
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > KMC Manipal > Obstetrics & Gynaecology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Manipal
Date Deposited: 05 Sep 2014 09:42
Last Modified: 17 Dec 2015 14:26
URI: http://eprints.manipal.edu/id/eprint/140549

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