Comparative sequence analysis of acid sensitive/resistance proteins in Escherichia coli and Shigella flexneri

Manikandan, Selvaraj and Balaji, S and Kumar, Anil and Kumar, Rita (2007) Comparative sequence analysis of acid sensitive/resistance proteins in Escherichia coli and Shigella flexneri. Bioinformation, An open access forum. ISSN 0973-2063

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Abstract

The molecular basis for the survival of bacteria under extreme conditions in which growth is inhibited is a question of great current interest. A preliminary study was carried out to determine residue pattern conservation among the antiporters of enteric bacteria, responsible for extreme acid sensitivity especially in Escherichia coli and Shigella flexneri. Here we found the molecular evidence that proved the relationship between E.coli and S.flexneri. Multiple sequence alignment of the gadC coded acid sensitive antiporter showed many conserved residue patterns at regular intervals at the N-terminal region. It was observed that as the alignment approaches towards the C-terminal, the number of conserved residues decreases, indicating that the Nterminal region of this protein has much active role when compared to the carboxyl terminal. The motif, FHLVFFLLLGG, is well conserved within the entire gadC coded protein at the amino terminal. The motif is also partially conserved among other antiporters (which are not coded by gadC) but involved in acid sensitive/resistance mechanism. Phylogenetic cluster analysis proves the relationship of Escherichia coli and Shigella flexneri. The gadC coded proteins are converged as a clade and diverged from other antiporters belongs to the amino acid-polyamine-organocation (APC) superfamily.

Item Type: Article
Uncontrolled Keywords: amino acid -polyamine-organocation (APC); Glutamate decarboxylase (GadC); bacteria; sequence; proteins
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 15 Sep 2014 07:22
Last Modified: 15 Sep 2014 07:22
URI: http://eprints.manipal.edu/id/eprint/140606

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