Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase

Kasinathan, Narayanan and Volety, Subrahmanyam Mallikarjuna and Rao, Venkata Josyula (2014) Application of Experimental Design in Preparation of Nanoliposomes Containing Hyaluronidase. Journal of Drug Delivery, 2014. pp. 1-7.

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Abstract

Hyaluronidase is an enzyme that catalyzes breakdown of hyaluronic acid. This property is utilized for hypodermoclysis and for treating extravasation injury. Hyaluronidase is further studied for possible application as an adjuvant for increasing the efficacy of otherdrugs.Development of suitable carrier system for hyaluronidase would helpinco administration of other drugs.Inthepresent study, the hyaluronidase was encapsulated in liposomes. The effect o f variables, namely, phosphatidylcholine (PC), cholesterol,temperature during film formation (T1 ), and speed of rotation of the flask during film formation (SPR) on percentage of protein encapsulation, was first analyzed using factorial design. The study showed that level of phosphatidylcholine had the maximum effect on the outcome. The effect of interaction of PC and SPR required for preparation of nanoliposomes was identified by central composite design (CCD). The dependent variables were percentage protein encapsulation, particle size, and zeta potential. The study showed that ideal conditions for production of hyaluronidase loaded nanoliposomes are PC—140mg and cholesterol 1/5th of PC when the SPR is 150 rpm and T1is 50∘C.

Item Type: Article
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Depositing User: KMC Manipal
Date Deposited: 12 Sep 2014 07:34
Last Modified: 12 Sep 2014 07:34
URI: http://eprints.manipal.edu/id/eprint/140625

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