Acute and Long Term Safety Evaluation of Grape Seed Extract and Zinc Containing Multivitamin-Mineral Nutritional Food Supplement in Rodents

Satyam, Shakta Mani and Bairy, KL (2014) Acute and Long Term Safety Evaluation of Grape Seed Extract and Zinc Containing Multivitamin-Mineral Nutritional Food Supplement in Rodents. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5 (5). pp. 561-566. ISSN 0975-8585

[img] PDF
185. Zincovit Toxocity.pdf - Published Version
Restricted to Registered users only

Download (670kB) | Request a copy

Abstract

To study the acute and sub-chronic toxicity associated with potential Zincovit tablets with grape seed extract among rats and mice. For acute toxicity in adult female Sprague-Dawley rats and Balb-c mice, the Acute Toxicity Class method (OECD 423 guideline) was employed. Animals were observed individually after dosing daily for a total of 14 days. Sub chronic toxicity was investigated in normal control (2% gum acacia, 1 ml/kg) and Zincovit tablets with grape seed extract at 50, 500 and 1250 mg/kg/day individually for 3 months in adult Sprague-Dawley female rats (4 groups, n= 6). Clinical signs, hematological and biochemical parameters were assessed. During the acute toxicity study, there were no behavioral changes or mortality observed even at 2000 mg/kg of Zincovit tablet with grape seed extract among rats and mice. There was no significant change in their body weight. LD50 cut-off for mice and rat was found to be above 5000 mg/kg and the drug came under the category 5 according to Globally Harmonized Classification System (OECD 423 guidelines). During the 90 days of sub-chronic toxicity study, treatment with combined formulation of grape seed extract and Zincovit tablets among Sprague-Dawley rats, the lowest-observed-adverse-effect levelsand no-observed-adverse-effect levels was observed as 500 mg/kg and 50 mg/kg respectively. The present study revealed that the single combined formulation of grape seed extract and Zincovit tablet is the potential functional nutritional food supplements that could offer a novel therapeutic opportunity against oxidative stress associated disorders in Sprague-Dawley rats.

Item Type: Article
Uncontrolled Keywords: Grape seed extract; Zincovit tablets; Antioxidants; Safety.
Subjects: Medicine > MMMC Manipal > Pharmacology
Medicine > KMC Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 17 Sep 2014 06:10
Last Modified: 17 Sep 2014 06:10
URI: http://eprints.manipal.edu/id/eprint/140670

Actions (login required)

View Item View Item