Enzymatic Modification of Chitosan Using Chitin Deacetylase Isolated from Bacillus cereus

Raval, Ritu and Raval, Keyur and Moerschbacher, BM (2013) Enzymatic Modification of Chitosan Using Chitin Deacetylase Isolated from Bacillus cereus. Open Access Scientific Reports, 2 (1). pp. 1-4.

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Official URL: http://dx.doi.org/10.4172/scientificreports.617

Abstract

One of the classes of polysaccharide with a promising economic potential comprises those with biological activities,such as chitosan, which has been optimised over the course of evolution to fulfil many roles in the life of bacteria, fungi and animals. However, there is still considerable leeway for optimisation of these biopolymers to fulfil their many roles in biotechnology. Today, modifications and optimisations are typically done using chemical methods such as acid or alkali treatment. Alternative and/or complementing enzymatic modifications using chitin deacetylase will reduce energy input and environmental impact while at the same time offering the advantage of higher specificity and, thus, potentially yielding novel polymers and/or oligomers with advanced physico-chemical properties and biological functionalities. In the following research work, one of the isolated Chitin Deacetylase (CDA) from Bacillus cereus was expressed in E. coli pLysS and later purified using Ni-NTA column. The purified product was later characterised for its pH specificity and co-factors.

Item Type: Article
Uncontrolled Keywords: Chitin deacetylase; E. coli plyss; Bacillus cereus; Enzyme purification
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 24 Sep 2014 04:21
Last Modified: 24 Sep 2014 04:21
URI: http://eprints.manipal.edu/id/eprint/140709

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