Antidiabetic effect of Ficus racemosa Linn. stem bark in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats: A mechanistic study

Veerapur, VP and Prabhakar, KR and Bansal, Punit and Srinivasan, KK and Unnikrishnan, MK (2012) Antidiabetic effect of Ficus racemosa Linn. stem bark in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats: A mechanistic study. Food Chemistry, 132. pp. 186-193.

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Abstract

The present study was designed to investigate the effects of the ethanol extract of Ficus racemosa (FRE) on biochemical parameters in type 2-like diabetes, induced by a combination of standardised high-fat diet and low-dose streptozotocin (25 mg kg�1, i.p.) in rats. To elucidate the mode of action of FRE, its effects on a battery of targets involved in glucose homeostasis was evaluated. FRE (200 and 400 mg kg�1, p.o.), in a dose-dependent manner, altered the biochemical parameters and significantly improved glucose tolerance and HDL-c levels. In different bioassays, FRE showed inhibition of PTP-1B (IC50 12.1 lg/mL) and DPPIV (42.5%). FRE exhibited 82.6% binding to PPAR-c. Furthermore FRE exhibited stimulation of glucose uptake by skeletal muscles (hemi-diaphragm). Bergenin was quantified in bioactive-FRE by high-performance liquid chromatography (0.15% w/w). This is the first report demonstrating the effectiveness of F. racemosa stem bark in type 2 diabetes and targets involved in it.

Item Type: Article
Uncontrolled Keywords: Ficus racemosa; High-fat diet-fed; STZ; DPP-IV; PPAR-c; PTB-1B; Glucose uptake; Bergenin.
Subjects: Pharmacy > MCOPS Manipal > Pharmacy Practice
Depositing User: KMC Manipal
Date Deposited: 20 Oct 2014 04:12
Last Modified: 20 Oct 2014 04:12
URI: http://eprints.manipal.edu/id/eprint/140893

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