Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach

Bansal, Punit and Paul, Piya and Unnikrishnan, MK (2012) Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach. Journal of Functional Foods, 4 (4). pp. 924-932.

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Abstract

The present study was aimed to evaluate the in vivo radioprotective efficacy of quercetin-3-O-rutinoside (PMC-1), the key bioactive constituent flavonoid glycoside isolated from the whole plant of Pilea microphylla was evaluated. In vivo survival studies established the optimum effective dose of PMC-1 at 25 mg/kg/i.p. At the optimum dose, PMC-1 prevented the depletion of endogenous antioxidants in the liver of irradiated mice. In vivo protection towards gastrointestinal tract and haematopoietic system was confirmed by the restoration of radiation-induced reduction in villi height, number of crypt cells and spleen index. PMC-1 also attenuated the radiation-induced apoptosis in spleenocytes significantly. Single cell gel electrophoresis of peripheral blood leukocytes showed inhibition of radiationinduced DNA damage by PMC-1. PMC-1 pretreatment significantly reversed the changes by increasing pro-survival (ERK) and decreasing pro-apoptotic (BAX) gene expressions compared to radiation control. Thus, PMC-1 exhibits protective effects against c-radiation and the probable mechanism of action involves maintenance of antioxidant enzymes, prophylactic action and inhibition of apoptosis.

Item Type: Article
Uncontrolled Keywords: Quercetin-3-O-rutinoside; flavonoid glycoside; radioprotection; DNA damage; apoptosis.
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Pharmacy > MCOPS Manipal > Pharmacy Practice
Depositing User: KMC Manipal
Date Deposited: 20 Oct 2014 04:18
Last Modified: 20 Oct 2014 04:18
URI: http://eprints.manipal.edu/id/eprint/140895

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