RAPD-PCR typing of Pseudomonas aeruginosa strains isolated from Peripheral Venous Catheters

Rao, Amita Shobha (2014) RAPD-PCR typing of Pseudomonas aeruginosa strains isolated from Peripheral Venous Catheters. Current Research in Microbiology and Biotechnology, 2 (5). pp. 462-465. ISSN 2320-2246

[img] PDF
CRMB Vol. 2, No. 5 (2014) 462-465.pdf - Published Version
Restricted to Registered users only

Download (311kB) | Request a copy

Abstract

The study aimed at genotyping Pseudomonas aeruginosa strains isolated from prolonged peripheral venous catheters of patients of three tertiary care hospitals in Mangalore, India by Random Amplified Polymorphic DNAPCR [RAPD-PCR] and find their clonal relatedness to phenotypic characteristics such as colony mucoidity and adherence ability. The P. aeruginosa isolates in this study were classified into five groups based on their phenotypic characteristics such as colony appearance [mucoid or non-mucoid] and degree of adherence [strong, morderate or non-adherent] to polystyrene culture plates. RAPD-PCR typing clustered the isolates studied into 19 genotypes at 73% similarity and discriminatory index of 0.91. Most of the genotypes were common to the three hospitals studied, indicating cross circulation of genotypes between hospitals. Genotyping of P.aeruginosa strains could not be associated to the phenotypic characteristics studied.

Item Type: Article
Uncontrolled Keywords: Pseudomonas aeruginosa ; peripheral venous catheter ; adherence ; RAPD.
Subjects: Medicine > MMMC Manipal > Microbiology
Depositing User: KMC Manipal
Date Deposited: 23 Oct 2014 06:27
Last Modified: 23 Oct 2014 06:27
URI: http://eprints.manipal.edu/id/eprint/140912

Actions (login required)

View Item View Item