MU Digital Repository
Logo

Effect of orexin B and OX2-R antagonist micro-infusion into the nucleus accumbens on consummatory behaviour in wistar rats

*, Rashmi KS and Mayannavar, Santosh and Deshpande, Keerthi and *, Ganaraja B (2014) Effect of orexin B and OX2-R antagonist micro-infusion into the nucleus accumbens on consummatory behaviour in wistar rats. European Journal of Biotechnology and Bioscience, 2 (4). pp. 30-36. ISSN 2321-9122

[img] PDF
Paper Published October Dr Ganaraja.pdf - Published Version
Restricted to Registered users only

Download (727kB) | Request a copy

Abstract

Aims and objectives: To elucidate role of Orexin B in the control of ingestive behaviour mediated through nucleus accumbens. Materials and Methods: In the present study Orexin B was infused into the nucleus accumbens in Wistar albino rats. Fifty four male Wistar rats, divided into three groups of 18 each, were further divided into three subgroups as Control, Sham operated control and experimental group (n=6 each). In the experimental group, Orexin B was administered bilaterally into the Nucleus accumbens in overnight fasted animals, at a dosage of 3nmol/μl (group 1) and 30nmol/μl (group 2) and their food intake and water intake were measured meticulously after 1 hour, 2 hours, 4hours, 6 hours,12 hours and 24 hours. Further to establish the role of Orexin B unequivocally, OX2R antagonist TCS-OX2-29 was infused into NAcc bilaterally in the third group and their feeding behaviour was assessed. Results: In low doses of Orexin B (3nm/μl) there was slight increase in food and water intake. But difference was not statistically significant, except increase in water intake at the end of first hour (p<0.05). The high dose of Orexin B (30nm/μl) increased food and water intake significantly at the end of 1, 2, 4, 6 hour, 12 hour and 24 hours, compared to control and sham operated rats. The infusion of the Orexin B antagonist (TCS-OX2-29) resulted in decreased food and water intake significantly at the end of 2, 4, 6, 12 hour and 24 hours.

Item Type: Article
Uncontrolled Keywords: Food intake, Nucleus accumbens, Orexin B, Orexin antagonist, Water intake.
Subjects: Medicine > KMC Mangalore > Physiology
Depositing User: KMCMLR User
Date Deposited: 29 Oct 2014 11:18
Last Modified: 29 Oct 2014 11:18
URI: http://eprints.manipal.edu/id/eprint/140944

Actions (login required)

View Item View Item