A micro-Raman study of live, single red bood cells (RBCs) treated with AgNO3 nanoparticles

Bankapur, Aseefhali and Barkur, Surekha and Chidangil, Santhosh and Mathur, Deepak (2014) A micro-Raman study of live, single red bood cells (RBCs) treated with AgNO3 nanoparticles. PLos One, 9 (7). pp. 1-10. ISSN 1932-6203

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Abstract

Silver nanoparticles (Ag NPs) are known to exhibit broad antimicrobial activity. However, such activity continues to raise concerns in the context of the interaction of such NPs with biomolecules. In a physiological environment NPs interact with individual biological cells either by penetrating through the cell membrane or by adhering to the membrane. We have explored the interaction of Ag NPs with single optically-trapped, live erythrocytes (red blood cells, RBCs) using Raman Tweezers spectroscopy. Our experiments reveal that Ag NPs induce modifications within an RBC that appear to be irreversible. In particular we are able to identify that the heme conformation in an RBC transforms from the usual R-state (oxy-state) to the T-state (deoxy-state). We rationalize our observations by proposing a model for the nanoparticle cytotoxicity pathway when the NP size is larger than the membrane pore size. We propose that the interaction of Ag NPs with the cell surface induces damage brought about by alteration of intracellular pH caused by the blockage of the cell membrane transport.

Item Type: Article
Subjects: Departments at MU > Atomic Molecular Physics
Depositing User: KMC Manipal
Date Deposited: 12 Nov 2014 04:28
Last Modified: 12 Nov 2014 04:28
URI: http://eprints.manipal.edu/id/eprint/141050

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