Oocytes recovered after ovarian tissue slow freezing have impaired H2AX phosphorylation and functional competence

Sam, Sudhakaran and Shubhashree, Uppangala and Salian, Sujith Raj and Honguntikar, Sachin D and Nair, Ramya and Kalthur, Guruprasad and Adiga, Satish Kumar (2014) Oocytes recovered after ovarian tissue slow freezing have impaired H2AX phosphorylation and functional competence. Reproduction, Fertility and Development.

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Abstract

It has been shown that oocytes isolated from ovarian tissue cryopreservation acquire DNA damage during the process of freeze–thawing. Using a mouse model, here we have investigated the functional competence and phosphorylation of H2AX (g-H2AX) in germinal vesicle (GV) and parthenogenetically activated oocytes derived from conventional ovarian tissue slow freezing and vitrification techniques. The number of GV-stage oocytes with g-H2AX foci was not significantly different between the slow-freezing and vitrification groups. Although the in vitro maturation (IVM) potential of GV oocytes in the slow-freezing group showed a significant delay (P,0.0001) in the process of germinal vesicle breakdown, no difference in the maturation rate was observed between the two protocols. Nevertheless,parthenogenetic activation of IVM oocytes using strontium chloride showed a significantly lower activation rate in the slow-freezing group compared with the vitrification (P,0.05) and control (P,0.01) groups. Importantly, H2AX phosphorylation was significantly perturbed in the slow-freezing group in comparison to the control (P,0.05). Therefore, we conclude that impaired sensing of DNA strand breaks and repair processes are associated with the reduced functional competence of the oocytes recovered from the slow-freezing group, which may have a significant impact on the reproductive outcome.

Item Type: Article
Uncontrolled Keywords: g-H2AX, in vitro maturation, vitrification.
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 14 Nov 2014 04:36
Last Modified: 14 Nov 2014 04:36
URI: http://eprints.manipal.edu/id/eprint/141076

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