Naha, Anup and Rai, Ikya and Kamath, Venkatesh B (2014) Development and validation of bioanalytical method for estimation of glibenclamide. Universal Journal of Pharmacy, 3 (5). pp. 42-45. ISSN 2320-303X

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Abstract

A simple, reliable, precise, rapid and reproducible reversed phase high performance liquid chromatographic method was developed and validated for the bioanalytical estimation of glibenclamide. Chromatographic separation was achieved isocratically with Grace Vydac C18 column (250 × 4.6 mm, 5m) and Acetonitrile: 20 mM ammonium acetate buffer, pH 4.5 (50:50) as mobile phase, at a flow rate of 1 ml/min with detection at 300 nm. Parameters such as selectivity, recovery, accuracy, precision, stability were studied as per ICH guidelines. The retention time for Glibenclamide and Internal standard, Glipizide was found to be 10.04 and 4.3 min and the peaks were of good shape and good separation and without any interferences. Linearity for glibenclamide was in the range between 0.2 – 20 ?g/mL and mean recoveries obtained for glibenclamide was 77.12 ± 3.21 %. Glibenclamide was stable in plasma for about one month when stored in the frozen state (-70o C). The developed method was found to be accurate, precise, selective and rapid for the bioanalytical estimation of glibenclamide.

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