Preparation and Characterization of Nanosuspension of Tamoxifen Citrate for Intra-Venous Administration in Drug Resistant Breast Cancer Cells

Upadhyay, Pooja and Koteshwara, KB (2014) Preparation and Characterization of Nanosuspension of Tamoxifen Citrate for Intra-Venous Administration in Drug Resistant Breast Cancer Cells. Advanced Science Letters, 20 (7). pp. 1483-1489.

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Abstract

Extensive studies performed here showed that Tamoxifen Citrate nanosuspension primed with several rigorous steps including pre-milling, magnetic stirring, sonication, high pressure homogenization was highly stable in its nano-forms and thus has been successfully nanonized. Optimization of the surfactant i.e., Tween 80® (Polysorbate 80) and stabilizer i.e., Pluronic F-68® (polaxamer188) ratio has been performed, and after optimization by employing several analytical techniques, the suitable ratio of surfactant:stabilizer for calculated dose of Tamoxifen Citrate was found to be 15% Tween 80 and 5% Polaxamer188. This quotient has been arrived at after considering the outcome of all the evaluations performed on the tamoxifen citrate nanosuspension (TEMParticle size, diffraction studies, FTIR-stability of drug molecule in the nanoformultion, Zeta potential-surface charge on molecules). Any chemical interactions amid the excipients and/or changes in the active therapeutic moiety were absent. It has been verified by performing FT-IR on the nano-formulations and Zeta potential of the nanosuspension was found to be 8.06 mV which is in the required range of Zeta Potential of +30 to −30 mV for nanoparticles suspended in Nanosuspension. Particle size of nano-formulation has been found to be ∼70 nm.This is idyllic for intra venous administration of the said drug formulation.

Item Type: Article
Uncontrolled Keywords: Tamoxifen Citrate; Tween 80; Polaxamer188; Nanosuspension; Intra Venous
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Manipal
Date Deposited: 06 Dec 2014 10:34
Last Modified: 06 Dec 2014 10:34
URI: http://eprints.manipal.edu/id/eprint/141231

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