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Evaluation of mast cells and eosinophils in odontogenic cysts: A histochemical approach

*, Astekar Madhusudan and Ahmed, Junaid and Metgud , Rashmi and *, Phull Khushboo and *, Sowmya GV (2014) Evaluation of mast cells and eosinophils in odontogenic cysts: A histochemical approach. Journal of Dr. NTR University of Health Sciences, 3 (4). pp. 243-248.

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Abstract

Background: Odontogenic cysts and tumors are most common benign destructive lesions in the oral cavity. These cystic lesions with its central fl uid, reservoir of non-physiological composition, is in itself likely to provoke an infl ammatory response in the surrounding host tissues, which may result in its formation and enlargement. Aims and Objectives: 1. To evaluate the number of mast cells and eosinophils in radicular cyst, keratocystic odontogenic tumor and dentigerous cyst and to intercompare it with the degree of infl ammation. 2. To evaluate and intercompare the staining quality of toluidine blue and thionin for mast cells identifi cation and Carbol Chromotrope and Congo red for eosinophils evaluation. Study Design: The study comprised of total 47 cases of which 20 were of radicular cyst, 15 were keratocystic odontogenic tumor and 12 were dentigerous cyst. Sections of 4μm thick were cut and stained with gold standard hematoxylin and eosin staining procedure for routine diagnosis. The amount of infl ammation in each cyst was graded. They were further stained with toluidine blue and thionin for mast cells identifi cation and Carbol Chromotrope and Congo red for e osinophils identifi cation. The statistical analysis used was one way ANOVA (Analysis of Variance) and Pearson’s Correlation Coeffi cient. Results: The mean number of cell count per high power fi eld of mast cells was higher than the eosinophils. On correlating with degree of infl ammation the results came out to be statistically signifi cant. The staining quality for mast cells was equally good with toluidine blue and thionin, whereas Carbol Chromotrope had a signifi cantly better staining quality than Congo red in case of eosinophils. Conclusion: The higher number of mast cells detected in odontogenic cysts could contribute in the release of infl ammatory mediators involved in the pathogenesis of odontogenic cysts formation and enlargement. The histochemistry using various stains has proved to be promising in identifi cation of mast cells and eosinophils.

Item Type: Article
Uncontrolled Keywords: Basophils, coloring agents, dentigerous cyst, eosinophils, keratocystic odontogenic tumor, mast cells, odontogenic cyst, radicular cyst, special stains, toluidine blue
Subjects: Dentistry > MCODS Mangalore > Oral Medicine and Radiology
Depositing User: KMCMLR User
Date Deposited: 24 Dec 2014 06:50
Last Modified: 24 Dec 2014 06:50
URI: http://eprints.manipal.edu/id/eprint/141334

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