Probing oxidative stress in single erythrocytes with Raman Tweezers.

Zachariah, E and Bankapur, A and Santhosh, C and Valiathan, Manna and Mathur, D (2010) Probing oxidative stress in single erythrocytes with Raman Tweezers. Journal of Photochemistry and Photobiology B: Biology, 100. pp. 113-116.

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Abstract

Raman Tweezers have been successfully applied to characterize chemically-induced oxidative stress on optically-trapped live, single erythrocytes. There is significant enhancement in Raman peak intensities corresponding to S@S and C–S stretching modes that are induced by oxidative stress. This is consistent with the formation of mixed disulphides between protein SH groups and low-molecular-mass thiols such as glutathione during oxidative damage to cells. Enhancement in glutathione level as a protective response against oxidative stress has been observed. Principal component analysis of the data yields good discrimination between spectra of normal and stress-induced red blood cells.

Item Type: Article
Uncontrolled Keywords: Raman Tweezers,Oxidative stress, Optical trap, Raman spectroscopy, Optical tweezers, Red blood cells.
Subjects: Departments at MU > Atomic Molecular Physics
Depositing User: KMC Manipal
Date Deposited: 30 Sep 2011 06:53
Last Modified: 18 Oct 2013 04:55
URI: http://eprints.manipal.edu/id/eprint/1415

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