Evaluation of high-performance liquid chromatography laser-induced fluorescence for serum protein profiling for early diagnosis of oral cancer.

Patil, Ajeetkumar and Prabhu, Vijendra and Choudhari, KS and Unnikrishnan, VK and George, Sajan D and Ongole, Ravikiran and Pai, Keerthilatha M and Shetty, Jayarama K and Bhat, Sujatha and Kartha, Vasudevan Bhaskaran and Chidangil, Santhosh (2010) Evaluation of high-performance liquid chromatography laser-induced fluorescence for serum protein profiling for early diagnosis of oral cancer. Journal of Biomedical Optics, 15 (6). pp. 1-10.

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Abstract

The present work deals with the evaluation of a high performance liquid chromatography laser-induced fluorescence(HPLCLIF)technique developed in our laboratory for early detection of oral cancer from protein profiles of body fluids. The results show that protein profiles of serum samples from a given class of samples, say, normal, premalignant, or malignant, are statistically very close to each other,while profiles of members of any class are significantly different from other classes. The performance of the technique is evaluated by the use of sensitivity and specificity pairs, receiver operating characteristic (ROC) analysis, and Youden’s Index. The technique uses protein profile differences in serum samples, registered by theHPLC-LIF technique. The study is carried out using serum samples from volunteers diagnosed as normal or premalignant clinically, and as malignant by histopathology. The specificities and sensitivities of the HPLC-LIF method at an ideal threshold (M-distance = 2) for normal, malignant, and premalignant classes are 100, 69.5, and 61.5%, and 86.5, 87.5, and 87.5% respectively.

Item Type: Article
Uncontrolled Keywords: oral cancer; serum; protein profile; receiver operating characteristics; Youden’s index, high-performance liquid chromatography, laser-induced fluorescence.
Subjects: Dentistry > MCODS Manipal > Oral Medicine and Radiology
Departments at MU > Atomic Molecular Physics
Health Sciences > KMCIC
Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Radiotherapy and Oncology
Depositing User: KMC Manipal
Date Deposited: 30 Sep 2011 04:15
Last Modified: 30 Sep 2011 04:15
URI: http://eprints.manipal.edu/id/eprint/1417

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