Assessment And Comparison of Keratin, Epithelial And Inflammatory Thickness In Oral Lichen Planus And Oral Lichenoid Reaction Using Picrosirius Red Stain

Pandya, Jay Ashokkumar and *, Srikant N and Boaz, Karen and Manaktala, Nidhi and Lewis, Amitha J and *, Supriya Nikita Kapila (2014) Assessment And Comparison of Keratin, Epithelial And Inflammatory Thickness In Oral Lichen Planus And Oral Lichenoid Reaction Using Picrosirius Red Stain. International Journal of Contemporary Medical Research, 1 (2). pp. 1-6.

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Abstract

Introduction: Lichen planus is a common, chronic autoimmune disorder of oral cavity. Other lesions often simulate oral lichen planus, most common being oral lichenoid reaction. In spite of having existent criteria defined by WHO, it often becomes difficult to sign out correct diagnosis. We hypothesized that Picrosirius red stain can aid to arrive at a correct diagnosis. Aim: We aimed to validate criteria like keratin and epithelial thickness as diagnostic parameters, which may help to differentiate between oral lichen planus and oral lichenoid reaction. Material and method: Fifteen cases of oral lichen planus and oral lichenoid reaction each, and ten cases of buccal mucosa as a comparison group were taken and stained with Picrosirius red stain. Epithelial, keratin and inflammatory thickness was measured used eye-piece reticule (10X magnification; Olympus CH20i, NA of 0.25). The thicknesses measured were then tabulated. The obtained thicknesses were compared using one way ANOVA test. The comparison within groups was made with Tukey-HSD test. Results: Significant differences were seen in thickness of keratin, epithelium and inflammation in lichen planus, lichenoid reaction and normal buccal mucosa (p value=0.002, <0.001 and <0.001 respectively). Keratin thickness and inflammatory component thickness were found to be maximum in lichenoid reaction, whereas epithelial thickness was measured highest in buccal mucosa. Conclusion: Picrosirius red stain can be used as an adjunct to routine haematoxylin and eosin stain for arriving at correct diagnosis in lichenoid lesions. Keratin and epithelial thickness can also be considered while differentiating lichenoid lesions.

Item Type: Article
Uncontrolled Keywords: Picrosirius red stain, keratin, epithelial and inflammatory thickness, oral lichen planus
Subjects: Dentistry > MCODS Mangalore > Oral Pathology
Depositing User: KMCMLR User
Date Deposited: 28 Jan 2015 10:20
Last Modified: 28 Jan 2015 10:20
URI: http://eprints.manipal.edu/id/eprint/141740

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