Phosphoproteome of Cryptococcus neoformans

Sharma, Jyothi and Pinto, Sneha M and Prasad, Keshava TS (2014) Phosphoproteome of Cryptococcus neoformans. Journal of Proteomics, 97. pp. 287-295. ISSN 1874-3919

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Cryptococcus neoformans is an encapsulated pathogenic yeast, which causes life threatening meningitis in immunocompromised individuals. C. neoformans var. grubii is the most prevalent and virulent form among the two varieties of C. neoformans - C. neoformans var. grubii and C. neoformans var. neoformans. The virulence of C. neoformans is mainly conferred by its capsule and melanin. cAMP dependent PKA-induced phosphorylation events are reported to be associated with the expression of these virulence traits, which highlights the importance of phosphoproteins in virulence and infection. Therefore, we performed global profiling of phosphoproteome of C. neoformans to enable a better understanding of molecular regulation of its virulence and pathogenesis. High resolution mass spectrometry of TiO2 enriched phosphopeptides from C. neoformans var. grubii grown in culture led to the identification of 1089 phosphopeptides derived from 648 proteins including about 45 kinases. Motif enrichment analysis revealed that most CDK family substrates were found to be phosphorylated. This indicates that cyclin-dependent kinases were among the active kinases in the pathogen in culture. These studies provide a framework for understanding virulence mechanisms in the context of signalling pathways in pathogenic yeast. This article is part of a Special Issue entitled: Trends in Microbial Proteomics.

Item Type: Article
Uncontrolled Keywords: Stress resonse ; Metal oxide affinity chromatography ; Fungal infection.
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Manipal
Date Deposited: 14 Feb 2015 04:44
Last Modified: 14 Feb 2015 04:44

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