Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach

Pinto, Sneha M (2014) Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach. EuPA Open Proteomics, 4. pp. 10-17. ISSN 2212-9685

[img] PDF
scan0029.pdf - Published Version
Restricted to Registered users only

Download (6MB) | Request a copy

Abstract

miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR.

Item Type: Article
Uncontrolled Keywords: Mass spectrometry ; miRNA targets ; HT-1080.
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Manipal
Date Deposited: 14 Feb 2015 05:08
Last Modified: 14 Feb 2015 05:08
URI: http://eprints.manipal.edu/id/eprint/141935

Actions (login required)

View Item View Item