Proteomics for understanding miRNA biology

Pinto, Sneha M (2013) Proteomics for understanding miRNA biology. Proteomics, 13 (3). pp. 558-567. ISSN 1615-9853

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Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in posttranscriptional regulation of gene expression. Mature miRNAs associate with the RNA interference silencing complex to repress mRNA translation and/or degrade mRNA transcripts. Mass spectrometry-based proteomics has enabled identification of several core components of the canonical miRNA processing pathway and their posttranslational modifications which are pivotal in miRNA regulatory mechanisms. The use of quantitative proteomic strategies has also emerged as a key technique for experimental identification of miRNA targets by allowing direct determination of proteins whose levels are altered because of translational suppression. This review focuses on the role of proteomics and labeling strategies to understand miRNA biology.

Item Type: Article
Uncontrolled Keywords: Cell biology ; iTRAO ; miRNA ; Multiple reaction monitoring ; noncoding RNA ; SILAC.
Subjects: Research > Research Center - Health Sciences
Depositing User: KMC Manipal
Date Deposited: 14 Feb 2015 05:14
Last Modified: 14 Feb 2015 05:14
URI: http://eprints.manipal.edu/id/eprint/141937

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