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Protein profile analysis of cellular samples from the cervix for the objective diagnosis of cervical cancer using HPLC-LIF.

Bhat, Sujatha and Patil, Ajeetkumar and Rai, Lavanya and Kartha, VB and Santhosh, C (2010) Protein profile analysis of cellular samples from the cervix for the objective diagnosis of cervical cancer using HPLC-LIF. Journal of Chromatography B, 878. pp. 3225-3230.

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Abstract

Chromatographic (HPLC) separation combined with ultra-sensitive laser induced fluorescence (LIF) detection. HPLC-LIF protein profiles of samples from clinically normal subjects, individuals suffering from cervical cancer (different stages), and subjects who had other gynecological problems related to cervix, like erosion of cervix and Nabothian cyst, but no malignancy, were subjected to Principal Component Analysis (PCA). The application of HPLC-LIF protein profiling combined with PCA was found to be a highly efficient method for discrimination of different classes of samples with high sensitivity and specificity. Diagnostic accuracy and optimal threshold – decision criterion – for objective discrimination were estimated using sensitivity–specificity pairs and Youden’s index (J) plots.

Item Type: Article
Uncontrolled Keywords: Cellular samples, HPLC-LIF,Principal Component Analysis Youden’s index.
Subjects: Departments at MU > Atomic Molecular Physics
Health Sciences > KMCIC
Medicine > KMC Manipal > Obstetrics & Gynaecology
Depositing User: KMC Manipal
Date Deposited: 30 Sep 2011 07:08
Last Modified: 30 Sep 2011 07:08
URI: http://eprints.manipal.edu/id/eprint/1421

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