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Isolation and Characterization of Filamentous Bacteria from A Full- Scale Municipal Wastewater Treatment Plant in Dubai

Ali Khan, Munawwar and S.M, Faheem (2012) Isolation and Characterization of Filamentous Bacteria from A Full- Scale Municipal Wastewater Treatment Plant in Dubai. International Conference on Environment, Chemistry and Biology, 49 (25).

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Abstract

This study aimed at isolating filamentous bacteria from the activated sludge samples of a fullscale municipal wastewater treatment plant in Dubai and studying their physiological and biochemical characteristics. Fluorescent in situ hybridization technique (FISH) was applied to identify filamentous bacteria pure culture isolates. In total, sixteen filamentous bacteria were successfully isolated and maintained on various culture media. Ten gram positive filamentous bacteria were successfully identified as nocardioform actinomycete using nocardioform group specific probes in FISH analysis. Most of the isolates were shown to utilize different carbon sources like glycerol, maltose, fructose etc. The optimum growth of most of the isolates was found to be at 27°C and neutral pH, however a few isolates were able to grow at 47°C and acidic pH. Overall results indicate that filamentous bacteria isolated in this study were quite diverse in their physiological and biochemical characteristics and could be of significance for further studies to establish their taxonomic status and possible role in foam formation and its control in the activated sludge process.

Item Type: Article
Subjects: Engineering > Dubai Campus > School of Life Sciences
Depositing User: Admin MU Dubai
Date Deposited: 18 Mar 2015 14:39
Last Modified: 22 Mar 2015 05:44
URI: http://eprints.manipal.edu/id/eprint/142219

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