Monitoring Microbial Diversity of a Full-Scale Municipal Wastewater Treatment Plant in Dubai

Ali Khan, Munawwar and Mohsin, Javeria and S.M, Faheem (2013) Monitoring Microbial Diversity of a Full-Scale Municipal Wastewater Treatment Plant in Dubai. APCBEE Procedia, 5. pp. 102-106.

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Abstract

In this study, fluorescence in situhybridization (FISH) technique was employedfor monitoring the microbial community in the activated sludge system of a full-scale municipal wastewater treatment plant in Dubai over a period of one year. A total of 96 activated sludge mixed liquor samples were characterized using ribosomal RNA (16S and 23S rRNA) targeted oligonucleotide probes for defined phylogenetic groups of bacteria. Several filamentous and non-filamentous bacteriawere predominantly found throughout the study period in all activated sludge mixed liquor samples. The bacterial species belonging to High G+C group were detected in both branched and single cell morphotypes. The previously published genus and species specific probes detected several of members belonging to archaea, sulphate reducing bacteria, ammonia-oxidizing bacteria, nitrobacter, nitrospira including halophilic and alotolerantnitrosomonasspp.In conclusion, the overall microbial community populations detected by the sub-group and species specific 16S and 23S rRNA targeted oligonucleotide probes found to be quite diverse and varied seasonally.

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