DNA Methylation Analysis of Phenotype Specific Stratified Indian Population

Rotti, Harish and Mallya, Sandeep and Kabekkodu, Shama Prasada and Chakrabarty, Sanjiban and Nair, Sreekumaran N and Valiathan, Marthanda VS and Satyamoorthy, K (2015) DNA Methylation Analysis of Phenotype Specific Stratified Indian Population. Journal of Translational Medicine, 13 (151). pp. 1-25. ISSN 1479-5876

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Abstract

Background DNA methylation and its perturbations are an established attribute to a wide spectrum of phenotypic variations and disease conditions. Indian traditional system practices personalized medicine through indigenous concept of distinctly descriptive physiological,psychological and anatomical features known as prakriti. Here we attempted to establish DNA methylation differences in these three prakriti phenotypes. Methods Following structured and objective measurement of 3416 subjects, whole blood DNA of 147 healthy male individuals belonging to defined prakriti (Vata, Pitta and Kapha) between the age group of 20-30years were subjected to methylated DNA immunoprecipitation (MeDIP) and microarray analysis. After data analysis, prakriti specific signatures were validated through bisulfite DNA sequencing.Results Differentially methylated regions in CpG islands and shores were significantly enriched in promoters/UTRs and gene body regions. Phenotypes characterized by higher metabolism (Pitta prakriti) in individuals showed distinct promoter (34) and gene body methylation (204), followed by Vata prakriti which correlates to motion showed DNA methylation in 52 promoters and 139 CpG islands and finally individuals with structural attributes (Kapha prakriti) with 23 and 19 promoters and CpG islands respectively. Bisulfite DNA sequencing of prakriti specific multiple CpG sites in promoters and 5′-UTR such as; LHX1 (Vata prakriti), SOX11 (Pitta prakriti) and CDH22 (Kapha prakriti) were validated. Kapha prakriti specific CDH22 5′-UTR CpG methylation was also found to be associated with higher body mass index (BMI).Conclusion Differential DNA methylation signatures in three distinct prakriti phenotypes demonstrate the epigenetic basis of Indian traditional human classification which may have relevance to personalized medicine.

Item Type: Article
Uncontrolled Keywords: DNA methylation; MeDIP; CpG Island; Prakriti; Ayurveda
Subjects: Departments at MU > Statistics
Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 22 May 2015 04:16
Last Modified: 22 May 2015 04:16
URI: http://eprints.manipal.edu/id/eprint/142710

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