IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells

Guruprasad, KP and Satyamoorthy, K (2015) IM-133N modulates cytokine secretion by RAW264.7 and THP-1 cells. Journal of Immunotoxicology. pp. 1-9. ISSN 1547-691X

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An indigenous herbal extract IM-133N containing extracts of Prosopis glandulosa Torr and Symplocos racemosa Roxb were evaluated for potential immunomodulatory effects using RAW264.7 and THP-1 cells. The incubation of the cells for 24 h with IM-133N over a dose range 0–125 mg/ml did not cause cytotoxicity that exceeded 10%. The results indicated that noncytotoxic doses of IM-133N effectively up-regulated iNOS, TNF�, IL-6, IL-10, IL-8 and IFN� gene expression in both the RAW264.7 and THP-1 cells. The results also indicated IM-133N elicited dose-related increases in nitric oxide (NO) and tumor necrosis factor (TNF)-� production by RAW264.7 or THP-1 cells. These results demonstrated that IM-133N could stimulate NO and induced pro-inflammatory cytokine expression by monocytes/macrophages. As clinical studies have shown IM-133N to be an effective immunomodulator without any adverse effects, the results of the present study provide further support for the potential use of this agent as an immunostimulant or as an immunotherapy adjuvant.

Item Type: Article
Uncontrolled Keywords: Adjuvant; herbal medicine; IFN�; immunostimulant; TNF�; interleukins.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 06 Jun 2015 11:39
Last Modified: 06 Jun 2015 11:39

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