Scanning electron microscopic in vitro study of a mineral drug against biofilm formation of enterococcus faecalis

Bernaitis, L and Sabarianandh, JV and Shenoy, Revathi P and Pai, Swathi (2014) Scanning electron microscopic in vitro study of a mineral drug against biofilm formation of enterococcus faecalis. International Journal of Health Information and Medical Research, 1 (4). pp. 3-7.

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Abstract

Background: E. faecalis is often found in root canal and lesions associated with periodontal diseases. The suppression and control of E. faecalis in dental procedures are paramount in limiting the invasion of bacteria into dentinal tubules and in abolishing the establishment of any relationship with other bacteria, as in biofilms. Aim: The main objective of this study was to evaluate the activity of mineral drug Palakarai Parpam, a Siddha drug against biofilm formation of E. faecalis. Materials and Methods: The anti biofilm effect of Palakarai Parpam and 5.25% Sodium Hypochlorite against E. faecalis has been studied by biofilm inhibition assay and scanning electron microscope (SEM). For biofilm inhibition assay, clinically isolated E. faecalis was grown in microtitre plates containing brain heart infusion broth and 5%, 10%drug and 5.25%sodium hypochlorite were added to each of the microtitre wells separately, After 10 minutes these wells were washed and the adherent biofilm was stained with 0.1% methylene blue and were determined at 490 nm in an ELISA reader. For SEM, sectioned teeth were obtained. Dental samples were processed. Biofilms formed on the tooth were dipped in to 5%drug, 10%drug and 5.25%sodium hypochlorite for 10 minutes. Samples were viewed and analysed under a Scanning Electron Microscopy. Results: Biofilm inhibition studies carried out using 10%drug and 5.25% sodium hypochlorite brought about complete inhibition of the biofilm (100%).In SEM the biofilm derived from 5% drug treatment reveals degeneration of biofilm with weakened cell masses. Palakarai parpam showed antimicrobial activity against the biofilm formation of E. faecalis.

Item Type: Article
Uncontrolled Keywords: Biofilm; siddha medicine; palakarai parpam; sodium hypochlorite.
Subjects: Medicine > KMC Manipal > Biochemistry
Dentistry > MCODS Manipal > Conservative Dentistry and Endontics
Medicine > KMC Manipal > Microbiology
Medicine > KMC Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 20 Jun 2015 06:16
Last Modified: 20 Jun 2015 06:16
URI: http://eprints.manipal.edu/id/eprint/143094

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