A pulse radiolysis study of hyperoside isolated from Hypericum mysorense

Raghu, Chandrasekhar H and Jagani, Hitesh (2015) A pulse radiolysis study of hyperoside isolated from Hypericum mysorense. Radiation Physicsand Chemistry, 107. pp. 149-159.

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Background:The recent growth in knowledge of free radicals in biology is producing a medical revolution that promises a new age in health and disease management. In the last two decades there has been an explosive interest in the role of oxygen free radicals, more generally known as “reactive oxygen species” and of “reactive nitrogen species” in experimental and clinical medicine. Methods:The flowering top extract of Hypericum mysorense possessing potent anti-oxidant activity was subjected to bio-active guided isolation. Pulse radiolysis technique was used to determine the transient spectrum and rate constant for the one-electron oxidation of hyperoside by View the MathML sourceOH, N3radical dotN3, NO2radical dotNO2, radical dotNONO, CCl3radical dotOOCCl3OO radicals in aqueous solution. Results:Three compounds were isolated and characterized as rutin, quercetin-3-O-galactoside (hyperoside) and quercetin from spectral analysis. The hyperoside radical showed pKa1 and pKa2 at 5.4 and 9.2. Both, Cu(II) and iron(II) ions form chelate with hyperoside. The Cu–hyperoside chelate was able to scavenge O2radical dot−O2−, k=7.0(±0.3)×106 dm3 mol−1 s−1 at pH 9. The repair rates for tryptophan and guanosine radicals by hyperoside were also determined. Conclusion:The reduction potential of hyperoside radical was determined by cyclic voltammetric and pulse radiolysis methods.

Item Type: Article
Uncontrolled Keywords: Hypericum mysorense; pulse radiolysis; Quercetin-3-O-galactoside; one-electron oxidation; hyperoside; free radical.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Biotechnology
Depositing User: KMC Manipal
Date Deposited: 06 Jul 2015 04:51
Last Modified: 06 Jul 2015 04:51
URI: http://eprints.manipal.edu/id/eprint/143255

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