Shirodkar, Rupesh and Shetty, Pallavi K and Kumar, Lalit and Mutalik, Srinivas and Lewis, Shaila Angela (2015) Development and validation of a reverse phase HPLC method for the estimation of lacidipine in nanoformulations. Latin American Journal of Pharmacy, 34 (6). pp. 1162-1166. ISSN 0326 2383
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Abstract
SUMMARY. The objective of the present study was to develop and validate a simple, accurate and reliable reverse phase high-performance liquid chromatography method for the estimation of lacidipine. The method was developed on Grace Vydac RP C-18 Column (250 × 4.6 mm i.d., particle size 5 μm) using mobile phase consisting in the ratio of 90:10 acetonitrile:0.1% triethylamine (TEA) solution (pH 6.5). The flow rate was maintained at 0.8 mL/min and effluent was monitored at 240 nm using UV detector. The retention time was found to be 7.4 min. Calibration curve was linear over the concentration range of 0.5-10 μg/mL. Intra- and inter-day precision % RSD values were found to be 0.541 and 0.828%, respectively. Recovery of lacidipine was in the range of 100.223-101.046 %. The limits of detection and quantification were 29.28 ng/mL and 88.73 ng/mL, respectively. The developed method was successfully applied for the quantitative determination of lacidipine in solid lipid nanoparticles.
Item Type: | Article |
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Uncontrolled Keywords: | Lacidipine; RP-HPLC; solid lipid nanoparticles; validation. |
Subjects: | Pharmacy > MCOPS Manipal > Pharmaceutics |
Depositing User: | KMC Manipal |
Date Deposited: | 06 Jul 2015 04:56 |
Last Modified: | 06 Jul 2015 04:56 |
URI: | http://eprints.manipal.edu/id/eprint/143257 |
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