Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines

Kanafi, Mohammad Mahboob and Mamidi, Murali Krishna and Sureshbabu, Shalini Kashipathi and Shahani, Pradnya and Bhawna, Chandravanshi and Warrier, Sudha R and Bhonde, Ramesh (2014) Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines. Biotechnology Letters, 36 (11). ISSN 0141-5492

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Abstract

To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: single cells and cellular aggregates. Subsequent analysis confirmed their identity as pancreatic acinar cells and islet-like cell aggregates (ICAs), as evidenced by amylase secretion and diphenylthiocarbazone staining respectively. Reverse transcriptase-PCR and immunocytochemistry assessment of the ICAs revealed the expression of pancreatic specific markers Ngn-3, Glut- 2, Pax-6 and Isl-1. These ICAs secreted insulin in response to glucose challenge, confirming their functionality. We propose that ICAs generated from HeLa and MCF-7 cell lines could form a promising in vitro platform of human islet equivalents (hIEQs) for diabetes research.

Item Type: Article
Uncontrolled Keywords: Breast cancer cell (MCF-7) ; Differentiation ; HeLa cells ; Human islet equivalents ; Islet-like cell aggregates.
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 14 Jul 2015 13:03
Last Modified: 14 Jul 2015 13:03
URI: http://eprints.manipal.edu/id/eprint/143343

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