Prevalence of Human Papillomavirus Types and Phylogenetic Analysis of HPV-16 L1 Var iants from Southern India

Kabekkodu, Shama Prasada and Bhat, Samatha and Pandey, Deeksha and Varghese, Vinay Koshy and Shukla, Vaibhav and Ghosh, Supriti and Kushtagi, Pralhad and Bhat, Parvati V and *, Puthiya Mundayat Gopinath and Satyamoorthy, K (2015) Prevalence of Human Papillomavirus Types and Phylogenetic Analysis of HPV-16 L1 Var iants from Southern India. Asian Pacific Journal of Cancer Prevention, 16 (5). pp. 2073-2080. ISSN 1513-7368

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Abstract

Background: The human papillomavirus (HPV) and its var iants show wide geogr aphical distr ibution and have been repor ted to cause cervical lesions. With cervical neoplasia as the leading cancer in Indian women, the aim of the present study was to evaluate the multiple infection HPV type distr ibution and var iant genotypes in cervical samples from the coastal Karnataka region, India. Mater ials and Methods: A total of 212 samples were screened by nested polymer ase chain reaction using PGMY9/11 and GP5+/6+ pr imer s. HPV positive samples were sequenced to identify the types and a phylogenetic tree was constr ucted using the neighbor-joining method. Results: Sequence analysis identiÀed a total of 14 HPV types distr ibuted in 20%, 73.3% and 82.5% of non-malignant, pre-malignant [low gr ade squamous intr aepithelial lesion (LSIL) and high gr ade squamous intr aepithelial lesion (HSIL)] and cervical cancer samples. The distr ibution of high r isk HPV in cancer samples was HPV 16, 76.4%, HPV18, 11.7%, HPV81, 2.9%, HPV31, 1.4%, HPV35, 1.4% and HPV 45, 1.4%. Multiple infections were observed in 11.8% of tumor samples with HPV 16 contr ibuting to 62.5% of cases. In non-malignant samples, 20% of HPV positive samples were detected with HPV16, 82.3%, HPV33, 5.8% and HPV58, 5.8% and very low incidence of multiple infections. Compar ative phylogenetic analysis of HPV var iants identiÀed 9 HPV sequences as new papillomavirus species, predominantly classiÀed as European lineage type. Conclusions: The Àndings for HPV infections associated with progression of cervical cancer in coastal Karnataka region and HPV var iant analysis provide baseline data for prevention and HPV vaccination progr ams.

Item Type: Article
Uncontrolled Keywords: Cervical cancer - human papillomavirus - multiple infections - nested PCR - PGMY9/11 - GP5+/6+
Subjects: Medicine > KMC Mangalore > Obstetrics & Gynaecology
Depositing User: KMCMLR User
Date Deposited: 20 Jul 2015 05:12
Last Modified: 04 Nov 2016 14:30
URI: http://eprints.manipal.edu/id/eprint/143450

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