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Effect of oxidized βB3-crystallin peptide (152–166) on thermal aggregation of bovine lens γ-crystallins: identification of peptide interacting sites

Udupa, Padmanabha EG (2005) Effect of oxidized βB3-crystallin peptide (152–166) on thermal aggregation of bovine lens γ-crystallins: identification of peptide interacting sites. Experimental Eye Research, 80 (2). pp. 185-196. ISSN 0014-4835

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Abstract

We studied the effect of oxidized βB3-crystallin peptide (residues 152–166) on the thermal aggregation of bovine γ-crystallin and on chaperone activity of α-crystallin. Thermal aggregation of γ-crystallin was higher in the presence of oxidized βB3-crystallin peptide than without oxidized peptide. Increased aggregation was not observed in the presence of unoxidized βB3-crystallin peptide or a control oxidized peptide. Enhanced aggregation of γ-crystallin by oxidized βB3-crystallin peptide was observed even at 37°C. Interaction with oxidized βB3-peptide increased the hydrophobicity in the γ-crystallin as shown by increased 4, 4′-dianilino-1, 1′-binaphthyl-5, 5′-disulfonic acid (bis-ANS) binding. Enhanced aggregation of γ-crystallin was observed despite the presence of α-crystallin (a chaperone protein) in the system. Sulfo succinimidyl-2-[6-(biotinamido)-2-{p-azidobenzamido}-hexanoamido]ethyl-1-3 dithio propionate (Sulfo-SBED) cross-linker studies further confirmed the interaction between oxidized βB3-crystallin peptide and γ-crystallin. Peptide interacted sites in γ-crystallin were identified by matrix assisted laser desorption time-of-flight mass spectrometric methods and the result suggests that oxidized βB3-crystallin peptide interacted with amino acid residues present on the outer surface of the γ-crystallin. These results imply that oxidized βB3-crystallin peptide interact with γ-crystallins and enhance their aggregation and light scattering.

Item Type: Article
Uncontrolled Keywords: Peptide; oxidation; γ-crystallin; thermal aggregation; chaperone activity; interaction; cross-linking; sulfo-SBED; photolysis.
Subjects: Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Manipal
Date Deposited: 05 Aug 2015 04:22
Last Modified: 05 Aug 2015 04:22
URI: http://eprints.manipal.edu/id/eprint/143742

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