Barkur, Surekha and Bankapur, Aseefhali and Chidangil, Santhosh (2015) Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy. Journal of Biomedical Optics, 20 (8). 085001-1. ISSN 1083-3668

PDF JBO_20_8_085001.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of O─P─O asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

Actions (login required)

View Item