Functional differences in mesenchymal stromal cells from human dental pulp and periodontal ligament

Vasandan, Anoop Babu and Shankar, Shilpa Rani and Prasad, Priya and Vulugundam, Sowmya Jahnavi and Bhonde, Ramesh and Susarla, Jyothi Prasanna (2014) Functional differences in mesenchymal stromal cells from human dental pulp and periodontal ligament. Journal of Cellular and Molecular Medicine, 18 (2). pp. 344-354.

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Abstract

Clinically reported reparative benefits of mesenchymal stromal cells (MSCs) are majorly attributed to strong immune-modulatory abilities not exactly shared by fibroblasts. However, MSCs remain heterogeneous populations, with unique tissue-specific subsets, and lack of clear-cut assays defining therapeutic stromal subsets adds further ambiguity to the field. In this context, in-depth evaluation of cellular characteristics of MSCs from proximal oro-facial tissues: dental pulp (DPSCs) and periodontal ligament (PDLSCs) from identical donors provides an opportunity to evaluate exclusive niche-specific influences on multipotency and immune-modulation. Exhaustive cell surface profiling of DPSCs and PDLSCs indicated key differences in expression of mesenchymal (CD105) and pluripotent/multipotent stem cell–associated cell surface antigens: SSEA4, CD117, CD123 and CD29. DPSCs and PDLSCs exhibited strong chondrogenic potential, but only DPSCs exhibited adipogenic and osteogenic propensities. PDLSCs expressed immuno-stimulatory/immune-adhesive ligands like HLA-DR and CD50, upon priming with IFNc, unlike DPSCs, indicating differential response patterns to pro- inflammatory cytokines. Both DPSCs and PDLSCs were hypo-immunogenic and did not elicit robust allogeneic responses despite exposure to IFNc or TNFa. Interestingly, only DPSCs attenuated mitogen-induced lympho-proliferative responses and priming with either IFNc or TNFa enhanced immuno-modulation capacity. In contrast, primed or unprimed PDLSCs lacked the ability to suppress polyclonal T cell blast responses. This study indicates that stromal cells from even topographically related tissues do not necessarily share identical MSC properties and emphasizes the need for a thorough functional testing of MSCs from diverse sources with respect to multipotency, immune parameters and response to pro-inflammatory cytokines before translational usage.

Item Type: Article
Uncontrolled Keywords: Mesenchymal stromal cells; dental pulp stromal cells; pro-inflammatory cytokines; � immune properties; periodontal ligament stromal cells; differentiation; stem cells.
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 08 Oct 2015 09:14
Last Modified: 08 Oct 2015 09:14
URI: http://eprints.manipal.edu/id/eprint/144343

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