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Detection of CTX-M, CTX-M 15, TEM and SHV type Extended Spectrum Beta lactamase genes among Gram negative clinical strains isolated from Government hospital, Mangalore

*, Neha and Routray, Abhisek and Shenoy, Shalini (2014) Detection of CTX-M, CTX-M 15, TEM and SHV type Extended Spectrum Beta lactamase genes among Gram negative clinical strains isolated from Government hospital, Mangalore. Journal of Pharmacy Research, 8 (5). pp. 614-618. ISSN 0974-6943

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Abstract

Aim: This study was carried out to evaluate the existence of the CTX-M, CTX-M 15, TEM and SHV genes in ESBL producing strains of Gram negative clinical isolates from Government Hospital, Mangalore, Karnataka (India). Materials and Methods: A total of 75 isolates were collected including family Enterobacteriaceae, Acinetobacter spp and Pseudomonas spp.The isolates were phenotypically confirmed as ESBL producers which were further evaluated for CTX-M, CTX-M 15, TEM and SHV genes by multiplex PCR. Results: Of the 75 isolates, PCR assay revealed that 51(68%), 3(4%), 21(28%) and22 (29.3%) were positive for CTX-M, CTX-M 15, TEM and SHV genes respectively. Conclusion: Molecular identification of the genes encoding beta lactamases would be essential for a reliable epidemiological investigation of their transmission in hospitals and antimicrobial resistance.

Item Type: Article
Uncontrolled Keywords: Cephalosporin, ESBLs, Gram negative bacteria,PCR
Subjects: Medicine > KMC Mangalore > Microbiology
Depositing User: KMCMLR User
Date Deposited: 27 Nov 2015 12:24
Last Modified: 27 Nov 2015 12:24
URI: http://eprints.manipal.edu/id/eprint/144620

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