Thymol, a Monoterpene Phenolic Derivative of Cymene, Abrogates Mercury-Induced Oxidative Stress Resultant Cytotoxicity and Genotoxicity in epatocarcinoma Cells

Shettigar, Nishan B and Das, Shubhankar and Rao, Nageshwar B and Rao, Satish BS (2015) Thymol, a Monoterpene Phenolic Derivative of Cymene, Abrogates Mercury-Induced Oxidative Stress Resultant Cytotoxicity and Genotoxicity in epatocarcinoma Cells. Environmental Toxicology, 30. pp. 968-980.

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Abstract

Thymol (TOH) was investigated for its ability to protect against mercuric chloride (HgCl2)- induced cytotoxicity and genotoxicity using human hepatocarcinoma (HepG2) cell line. 3-(4,5-Dimethylthiazol- 2-yl)22,5-diphenyl tetrazolium bromide assay confirmed the efficacy of TOH pretreatment in attenuating HgCl2-induced cytotoxicity. Pretreatment with TOH inhibited HgCl2-induced genotoxicity, depolarization of mitochondrial membrane, oxidative stress, and mitochondrial superoxide levels. Interestingly, TOH (100 mM) alone elevated the intracellular basal glutathione S-transferase (GST) levels and TOH pretreatment abrogated the decrease in glutathione, GST, superoxide dismutase, and catalase levels even after HgCl2 intoxication. Furthermore, TOH was also capable of inhibiting HgCl2-induced apoptotic as well as necrotic cell death analyzed by flowcytometric analysis of cells dual stained with Annexin- FITC/propidium iodide. The present findings clearly indicate the cytoprotective potential of TOH against HgCl2-induced toxicity, which may be attributed to its free radical scavenging ability which facilitated in reducing oxidative stress and mitochondrial damage thereby inhibiting cell death.

Item Type: Article
Uncontrolled Keywords: Mercury; Thymol; Genotoxicity; Apoptosis
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 07 Dec 2015 09:30
Last Modified: 07 Dec 2015 09:30
URI: http://eprints.manipal.edu/id/eprint/144704

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