Prediction of Absorption Coefficients by Pulsed Laser Induced Photoacoustic Measurements

Mallika, Priya and Rao, Satish BS and Ray, Satadru and Mahato, KK (2014) Prediction of Absorption Coefficients by Pulsed Laser Induced Photoacoustic Measurements. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 127. pp. 85-90.

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Abstract

In the current study, a pulsed laser induced photoacoustic spectroscopy setup was designed and developed, aiming its application in clinical diagnostics. The setup was optimized with carbon black samples in water and with various tryptophan concentrations at 281 nm excitations. The sensitivity of the setup was estimated by determining minimum detectable concentration of tryptophan in water at the same excitation, and was found to be 0.035 mM. The photoacoustic experiments were also performed with various tryptophan concentrations at 281 nm excitation for predicting optical absorption coefficients in them and for comparing the outcomes with the spectrophotometrically-determined absorption coefficients for the same samples. Absorption coefficients for a few serum samples, obtained from some healthy female volunteers, were also determined through photoacoustic and spectrophotometric measurements at the same excitations, which showed good agreement between them, indicating its clinical implications.

Item Type: Article
Uncontrolled Keywords: Photoacoustic spectroscopy; PAS setup; Absorption coefficient; Tryptophan; Serum; Spectrophotometry
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Surgical Oncology
Depositing User: KMC Library
Date Deposited: 07 Dec 2015 09:41
Last Modified: 07 Dec 2015 09:41
URI: http://eprints.manipal.edu/id/eprint/144711

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