Trends in clonal diversity and mating type allele analysis of pathogenic cryptococcus from southern Karnataka

Peralam Yegneswaran, Prakash and Rao, PS (2012) Trends in clonal diversity and mating type allele analysis of pathogenic cryptococcus from southern Karnataka. Advances in Life Sciences, 10. pp. 195-201.

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Abstract

Cryptococcus neoformans is a eukaryotic, heterobasidiomycetous yeast;pathogenic to mammalian hosts with an epiphytic and saprophytic modeof existence in the environment. The present study explores the diversity among the pathogenic population of Cryptococcus neoformans serotypeA and to investigate mode of reproduction and degree of clonality intheir population structure in the geographic settings of southern India.In vitro crossing experiments were performed using the congenic strainsJEC20 (serotype D, MATa) and JEC21 (serotype D, MATá) are used astesters. These two strains differ only at the mating type locus. Thedetermination of mating type was analyzed using the two gene loci STE12 and STE20 PCR primer pairs, specific for mating type á and a. Results reveals that different regions of the genome have different phylogenetichistories with improved hybrid fitness.

Item Type: Article
Uncontrolled Keywords: Cryptococcus; Mating type allele; Clonal population; Meningitis.
Subjects: Medicine > KMC Manipal > Microbiology
Depositing User: KMC Library
Date Deposited: 16 Dec 2015 11:30
Last Modified: 16 Dec 2015 11:30
URI: http://eprints.manipal.edu/id/eprint/144815

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