Selective cytotoxicity and pro-apoptotic activity of stem bark of Wrightia tinctoria (Roxb.) R. Br. in cancerous cells

Chaudhary, Shilpee and Devkar, Raviraj Anand and Bhere, Deepak and Setty, Manjunath M and Pai, Sreedhara Ranganath K (2015) Selective cytotoxicity and pro-apoptotic activity of stem bark of Wrightia tinctoria (Roxb.) R. Br. in cancerous cells. Pharmacognosy Magazine, 11 (44). S-431. ISSN 0973-1296

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Abstract

Background: Wrightia tinctoria (Roxb.) R. Br. is a widely available shrub in India used traditionally in various ailments, including cancer. However, the anticancer activity of the bioactive fractions has not been validated scientifically. Objective: To investigate the anticancer potential of stem bark of W. tinctoria and establish its phytochemical basis. Materials and Methods: The ethanol extract and subsequent fractions, petroleum ether, ethyl acetate, n-butanol, and aqueous were prepared by standard methods. In vitro cytotoxicity was determined in MCF-7 (breast) and HeLa (cervical) adenocarcinoma cells, and V79 (nontumor fibroblast) cells and apoptogenic activity in MCF-7 cells by acridine orange (AO)/ethidium bromide (EB) staining. Additionally, the antioxidant potential was evaluated using suitable methods. High-performance thin layer chromatography (HPTLC) analysis was performed for identification of active phytoconstituents. Results: Petroleum ether and ethyl acetate fractions were most potent with IC50values of 37.78 and 29.69 μg/ml in HeLa and 31.56 and 32.63 μg/ml in MCF-7 cells respectively in the sulforhodamine B assay. Comparable results were obtained in HeLa cells in 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide assay and interestingly, the fractions were found to be safe to noncancerous fibroblast cells. Both fractions induced significant (P < 0.05) apoptotic morphological changes observed by AO/EB staining. Moreover, extract/fractions exhibited excellent inhibition of lipid peroxidation with the ethyl acetate fraction being most active (IC50: 23.40 μg/ml). HPTLC confirmed the presence of two anti-cancer triterpenoids, lupeol, and β-sitosterol in active fractions. Conclusion: Extract/fractions of W. tinctoria exhibit selective cytotoxicity against cancerous cells that is mediated by apoptosis. Fractions are less toxic to noncancerous cells; hence, they can be developed as safer chemopreventive agents.

Item Type: Article
Uncontrolled Keywords: β-sitosterol; anticancer; chemoprevention; high-performance thin layer chromatography; lupeol.
Subjects: Pharmacy > MCOPS Manipal > Pharmacognosy
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 21 Dec 2015 09:05
Last Modified: 21 Dec 2015 09:05
URI: http://eprints.manipal.edu/id/eprint/144884

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