Reticulum vs Inclusions: A Learning Experience in Haemoglobin H Disease

*, Sridevi H.B. and Hegde, Anupama and *, Prashantha B. and Khadilkar, Urmila N and Chakraborti, Shrijeet (2015) Reticulum vs Inclusions: A Learning Experience in Haemoglobin H Disease. Journal of Clinical and Diagnostic Research, 9 (10). ED17-ED19. ISSN 0973-709X

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Haemoglobin H disease, also known as the alpha-thalassaemia is characterized by the presence of HbH inclusions in red blood cells, detectable on supra-vital stain. We present a case of a previously asymptomatic 31-year-old male, who insidiously developed anaemia and had prominent splenomegaly. Peripheral smear examination revealed microcytic hypochromic anaemia with numerous spherocytes and moderate polychromasia. In reticulocyte preparation with Brilliant cresyl blue, HbH inclusions were mistakenly identified as granulofilamentous reticulum of reticulocytes, giving a spuriously high reticulocyte percentage. After the literature review, repeat assessment was performed and with the aid of high performance liquid chromatography result, it was possible to delineate the HbH inclusions.

Item Type: Article
Uncontrolled Keywords: Brilliant cresyl blue, Granulofilamentous reticulum, HbH inclusions, High performance liquid chromatography
Subjects: Medicine > KMC Mangalore > Pathology
Medicine > KMC Mangalore > Biochemistry
Medicine > KMC Mangalore > Medicine
Depositing User: KMCMLR User
Date Deposited: 06 Jan 2016 12:11
Last Modified: 06 Jan 2016 12:11

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