Ethanolic Neem (Azadirachta indica) Leaf Extract Prevents Growth of MCF-7 and HeLa Cells and Potentiates the Therapeutic Index of Cisplatin

Sharma, Chhavi and Andrea, J. Vas and Payal, Goala and Taher, M Geewala and Tahir, A. Rizvi and Hussain, Arif (2014) Ethanolic Neem (Azadirachta indica) Leaf Extract Prevents Growth of MCF-7 and HeLa Cells and Potentiates the Therapeutic Index of Cisplatin. Journal of Oncology.

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Abstract

Thepresent study was designed to gain insight into the antiproliferative activity of ethanolic neem leaves extract (ENLE) alone or in combinationwith cisplatin by cell viability assay on human breast (MCF-7) and cervical (HeLa) cancer cells.Nuclearmorphological examination and cell cycle analysis were performed to determine the mode of cell death. Further, to identify its molecular targets, the expression of genes involved in apoptosis, cell cycle progression, and drug metabolism was analyzed by RT-PCR. Treatment of MCF-7, HeLa, and normal cells with ENLE differentially suppressed the growth of cancer cells in a dose- and time-dependent manner through apoptosis. Additionally, lower dose combinations of ENLE with cisplatin resulted in synergistic growth inhibition of these cells compared to the individual drugs (combination index <1). ENLE significantlymodulated the expression of bax, cyclin D1, and cytochrome P450 monooxygenases (CYP 1A1 and CYP 1A2) in a time-dependent manner in these cells. Conclusively, these results emphasize the chemopreventive ability of neem alone or in combination with chemotherapeutic treatment to reduce the cytotoxic effects on normal cells, while potentiating their efficacy at lower doses.Thus, neem may be a prospective therapeutic agent to combat gynecological cancers.

Item Type: Article
Subjects: Engineering > Dubai Campus > School of Life Sciences
Depositing User: Admin MU Dubai
Date Deposited: 22 Mar 2016 15:41
Last Modified: 22 Mar 2016 15:41
URI: http://eprints.manipal.edu/id/eprint/145630

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