PEGylated liposomes of anastrozole for long-term treatment of breast cancer: in vitro and in vivo evaluation

Shavi, Gopal Venkatesh and Reddy, Meka Sreenivasa and Nayak, Usha Yogendra and Deshpande, Praful Balavant and Udupa, N and Dave, Vivek (2016) PEGylated liposomes of anastrozole for long-term treatment of breast cancer: in vitro and in vivo evaluation. Journal of Liposome Research, 26 (1). pp. 28-46. ISSN 0898-2104

[img] PDF
469 - DisplayPdf.pdf - Published Version
Restricted to Registered users only

Download (2MB) | Request a copy


The aim of present study was to develop conventional and PEGylated (long circulating), liposomes containing anastrozole (ANS) for effective treatment of breast cancer. ANS is a thirdgeneration non-steroidal aromatase inhibitor of the triazole class used for the treatment of advanced and late-stage breast cancer in post-menopausal women. Under such disease conditions the median duration of therapy should be prolonged until tumor regression ends (431 months). Liposomes were prepared by the thin film hydration method by using ANS and various lipids such as soyaphosphatidyl choline, cholesterol and methoxy polyethylene glycol distearoyl ethanolamine in different concentration ratios and evaluated for physical characteristics, in vitro drug release and stability. Optimized formulations of liposome were studied forin vitro cytotoxic activity against the BT-549 and MCF-7 cell lines and in vivo behavior in Wistar rats. Preformulation studies, both Fourier transform infrared study and differential scanning calorimetry analysis showed no interaction between the drug and the excipients used in the formulations. The optimized formulations AL-07 and AL-09 liposomes showed encapsulation efficiencies in the range 65.12 ± 1.05% to 69.85 ± 3.2% with desired mean particle size distribution of 101.1 ± 5.9 and 120.2 ± 2.8nm and zeta potentials of �43.7 ± 4.7 and �62.9 ± 3.5 mV. All the optimized formulations followed Higuchi-matrix release kinetics and when plotted in accordance with the Korsemeyer–Peppas method, the n-value 0.55n51.0 suggests an anomalous (non-Fickian) transport. Likewise, the PEGylated liposomes showed greater tumor growth inhibition on BT-549 and MCF-7 cell lines from in vitro cytotoxicity studies (p50.05). Pharmacokinetic st dy of conventional and PEGylated liposomes in Wistar rats demonstrated a 3.33- and 20.28-fold increase in AUC(0–1) values when compared to pure drug (p50.001). Among the formulations, PEGylated liposomes showed encouraging results by way of their long circulation and sustained delivery properties for effective treatment of breast cancer.

Item Type: Article
Uncontrolled Keywords: Anastrozole; breast cancer; chemotherapy; cytotoxic; PEGylated liposomes.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 14 Apr 2016 10:14
Last Modified: 14 Apr 2016 10:14

Actions (login required)

View Item View Item