Radiation and Chemical Mutagen Induced Somaclonal Variations through in Vitro Organogenesis of Cotton (Gossypium hirsutum L.)

Muthusamy, A (2014) Radiation and Chemical Mutagen Induced Somaclonal Variations through in Vitro Organogenesis of Cotton (Gossypium hirsutum L.). International Journal of Radiation Biology, 90 (12). pp. 1229-1239. ISSN 1362-3095

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Abstract

Purpose : The purpose of the investigation was to induce somaclonal variations by gamma rays (GR), ethylmethane sulphonate (EMS) and sodium azide (SA) during in vitro organogenesis of cotton.Materials and methods : The shoot tip explants were irradiated with 5 – 50 Gray (Gy) GR (Cobalt 60), 0.5 – 5.0 mM EMS and SA separately, and inoculated on Murashige and Skoog (MS) medium fortifi ed with plant growth regulator (PGR) for organogenesis.The plantlets with well-developed root systems were acclimatized and transferred into the experimental fi eld to screen the somaclonal variations during growth and development.Results : The number of somaclonal variations was observed in growth of irradiated/treated shoot tips, multiplication, plantlet regeneration and growth in vitro and ex vitro. The lower doses/concentrations of mutagenic treatments showed signifi cant enhancement in selected agronomical characters and they showed decreased trends with increasing doses/concentrations of mutagenic agents.Conclusions : The re ults of the present study revealed the influence of lower doses/concentrations of mutagenic treatments on in vitro and ex vitro growth of cotton plantlets and their signifi cant improvement in agronomical characters which needs further imperative stability analysis. The present observations showed the platform to use lower doses/concentrations of mutagenic agents to induce variability for enhanced agronomical characters, resistant and tolerant cotton varieties.

Item Type: Article
Uncontrolled Keywords: Cotton; Mutagenesis; Plantlets; variations; Performance; Characters
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Library
Date Deposited: 21 Apr 2016 08:56
Last Modified: 11 Nov 2016 08:35
URI: http://eprints.manipal.edu/id/eprint/145900

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