A Comparative Study of the Release of Indomethacin from Chitosan Based Cross-linked Hydrogels

Goyal, A S and Divya, . and Kamath, R R and Thakur, Goutam (2016) A Comparative Study of the Release of Indomethacin from Chitosan Based Cross-linked Hydrogels. In: Belgian Symposium on Tissue Engineering, 14-15 April 2016, Leuven, Belgium.

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Abstract

A comparative study of the release of Indomethacin from chitosan based hydrogels has been carried out using biopolymers viz. gelatin and chitosan which are efficiently biocompatible with biological fluids and cells [1-2]. Genipin, a natural cross-linker with negligible toxicity, which is obtained from the gardenia fruit [3], was extensively used for comparing hydrogels. Hydrogels have been used as scaffolds in controlled drug delivery systems for various biomedical applications [3]. Chitosan and chitosan-gelatin composite hydrogels were formulated and cross-linked with 1% (w/v) genipin solution. The above mentioned hydrogels were characterized by subjecting them to swelling by placing the hydrogels in water and calculating their weight; compression was done by placing the hydrogels between two plates and calculating the compressive modulus and the microstructure was studied using the Scanning Electron Microscope (SEM). The release studies of Indomethacin (model drug) from gel matrices were performed in deionized water. The amount of drug released in the DI water at set time intervals over a period of 24h was analysed at 317nm using a spectrophotometer and the percentage release was calculated. All the experiments were done in triplicate (n=3). The composite hydrogels had a swelling index between 200-300% whereas the chitosan hydrogels had swelling indices above 400% which was analysed for over a period of 5 hours. The percentage release of indomethacin from the composite hydrogels was around 250%, whereas for chitosan hydrogels, it was above 400% for over a period of 24 hours. Chitosan- genipin hydrogels had a compressive modulus of 0.0347 ± 0.01 MPa while composite-genipin hydrogels had a compressive modulus of 0.1539 ± 0.02 MPa. SEM analysis provided the microstructural information of these hydrogels. The composite hydrogels have a better controlled release of drugs as suggested by the swelling index and the drug release profile. The compression test suggests that the composite hydrogels have a higher compressive modulus than the chitosan hydrogels. Finally, the SEM results indicate that the surface of the composite hydrogel is compact.

Item Type: Conference or Workshop Item (Paper)
Subjects: Engineering > MIT Manipal > Biomedical
Depositing User: MIT Library
Date Deposited: 26 Apr 2016 10:41
Last Modified: 26 Apr 2016 10:41
URI: http://eprints.manipal.edu/id/eprint/145920

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