Development and performance evaluation of novel nanoparticles of agrafted copolymer loaded with curcumin

Mutalik, Srinivas and Suthar, Neelam A and Managuli, Renuka S and Shetty, Pallavi K (2016) Development and performance evaluation of novel nanoparticles of agrafted copolymer loaded with curcumin. International Journal of Biological Macromolecules, 86. pp. 709-720. ISSN 0141-8130

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Abstract

Inflammatory bowel disease (IBD) is an inflammatory condition with mucosal ulceration, edema andhemorrhage of gastrointestinal tract. Curcumin has been shown to mitigate colitis in animal models.However, its usefulness is reduced due to poor pharmacokinetic behavior and low oral bioavailabil-ity. To address this, novel pH-sensitive hydrolyzed polyacrylamide-grafted-xanthan gum (PAAm-g-XG)nanoparticles (NPs) loaded with curcumin were prepared for colonic delivery. Optimized nanoparticles(CN20) were spherical, with an average size of 425 nm. A negligible amount of curcumin (≈8%) wasreleased from CN20 NPs in pH 1.2 and 4.5 solutions. When the pH was increased to 7.2, curcumin releasewas comparatively faster than that observed with pH 1.2 and 4.5 collectively. In pH 6.8 solution, excellentrelease of curcumin was observed. Highest curcumin release was observed when rat caecal contents wereincorporated in pH 6.8 solution, indicating microflora-dependent drug release property of NPs. In aceticacid-induced IBD in rats, curcumin NPs reduced myeloperoxidase and nitrite levels, prevented weightloss and attenuated colonic inflammation. Curcumin was better absorbed systemically in nanopartic-ulate form with increased Cmax(∼3 fold) and AUC (∼2.5 fold) than when delivered as free curcumin.We demonstrate successful development of grafted co-polymeric NPs containing drug suitable for colontargeting.

Item Type: Article
Uncontrolled Keywords: Grafted copolymer; polyacrylamide; xanthan gum; nanoparticles; inflammatory bowel disease; curcumin.
Subjects: Medicine > KMC Manipal > Obstetrics & Gynaecology
Pharmacy > MCOPS Manipal > Pharmaceutics
Depositing User: KMC Library
Date Deposited: 24 May 2016 09:20
Last Modified: 24 May 2016 09:20
URI: http://eprints.manipal.edu/id/eprint/146136

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